This is the property that gives CRISPR (Clustered Regularly Interspaced Short Palindromic Repeats) its tongue-twisting name. When the complementary strand is read backwards, the sequence is 5’-GGATCC-3’ which is identical to the first one, making it a palindromic sequence. In palindromic DNA-sequences, the letters of the genetic code, the four base molecules adenine, cytosine, thymine and guanine, are ordered such that they have the same order as the second complementary DNA-strand in this case read in opposite direction. This is the sequence where the restriction endonuclease, BamHI, binds to and cleaves at a specific cleavage site. An example of a palindromic sequence is 5’-GGATCC-3’, which has a complementary strand, 3’-CCTAGG-5’. An example of a palindromic sequence is 5-GGATCC-3, which has a complementary strand, 3-CCTAGG-5. The complement to the whole six base strand is. Note the first three bases GGA are followed by the complement of those three bases in reverse order: TCC. For example the recognition sequence for BamHI is GGATCC. An example of this is shown in the diagram. Palindromic sequences are a short run of bases (typically 3 to 5 in length), follow by their complementary bases in reverse order. Some restriction enzymes recognize a section of DNA that is the same sequence read 53 on one strand as it is read 53 on the complementary strand. So if a sequence is palindromic, the nucleotide sequence of one strand would be the same as its reverse complementary strand. A palindromic sequence is a sequence made up of nucleic acids within double helix of DNA and/or RNA that is the same when read from 5 to 3 on one strand and 5 to 3 on the other, complementary, strand. Question Video: Recognizing Palindromic Sequences. The pairing of nucleotides within the DNA double-helix is complementary which consist of Adenine (A) pairing with either Thymine (T) in DNA or Uracil (U) in RNA, while Cytosine (C) pairs with Guanine (G). The RNA sequence UAGCUA is genetic palindrome because, read backwards, it reads AUCGAU, the complement to the original sequence. Recognition sites of many restriction enzymes are palindromic. example, the DNA sequence ACCTAGGT is palindromic because its nucleotide-by-nucleotide complement is TGGATCCA, and reversing the order of the nucleotides in the complement gives the original sequence. The sequence is the same when one strand is read left to right and the other strand is read right to left. In molecular biology, palindromic sequences are referred to as the sequence of nucleotides in the DNA duplex or RNA, where the sequence in one strand is the same as the complementary sequence of the other strand when read from the same direction on both the strands, either 5’ to 3’ or 3’ to 5’. A DNA sequence whose 5'-to-3' sequence is identical on each DNA strand.
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